Ese dihydrocoumarin ni uburozi

Dihydrocoumarin, impumuro nziza, ikoreshwa mu biribwa, ikoreshwa kandi nk'insimbura coumarin, ikoreshwa nk'uburyohe bw'ubwiza; Amavuta avanze, uburyohe bwa kokonati, sinamoni; Ikoreshwa kandi nk'uburyohe bw'itabi.

Ese dihydrocoumarin ni uburozi

Dihydrocoumarin ntabwo ari uburozi. Dihydrocoumarin ni ikintu karemano kiboneka mu mpundu z'umuhondo za vanilla. Itegurwa hifashishijwe hydrogenation ya coumarin mu gihe hari catalyst ya nickel kuri 160-200 ℃ kandi iri munsi y'umuvuduko. Ishobora kandi gukoreshwa nk'ibikoresho fatizo, igashyirwamo hydrolyze mu gisubizo cy'amazi cya alkaline kugira ngo ikore aside o-hydroxyphenylpropionic, amazi ahagije, kandi ifunguye.

Imiterere y'ububiko

Bifunze kandi byijimye, bibitswe ahantu hakonje kandi humutse, umwanya uri mu icupa ni muto uko bishoboka kose hakurikijwe uruhushya rw'umutekano, kandi byuzuyemo uburinzi bwa azote. Bika mu bubiko bukonje kandi bufite umwuka mwiza. Ntukagire aho umuriro n'amazi bihurira. Bigomba kubikwa ukwabyo n'umusemburo, ntuvange ububiko. Bifite ubwoko n'ingano y'ibikoresho bizimya umuriro.

Inyigo yakozwe muri vitro

Isuzuma rya "in vitro enzymatic assay", dihydrocoumarin yateje ikumirwa rya SIRT1 (IC50 ya 208μM) rishingiye ku bwinshi. Kugabanuka kw'imikorere ya SIRT1 deacetylase byagaragaye ndetse no ku gipimo cya micromolar (85±5.8 na 73± 13.7% kuri 1.6μM na 8μM, uko bikurikirana). Microtubule SIRT2 deacetylase nayo yakumiriwe mu buryo bumwe bushingiye ku gipimo (IC50 ya 295μM).

Nyuma y'amasaha 24 yo gukubitwa, dihydrocoumarin (1-5mM) yongereye uburozi mu turemangingo twa TK6 mu buryo bushingira ku gipimo. Dihydrocoumarin (1-5mM) yongereye apoptosis mu turemangingo twa TK6 mu buryo bushingira ku gipimo mu masaha 6. Igipimo cya 5mM cya dihydrocoumarin cyongereye apoptosis mu masaha 6 mu turemangingo twa TK6. Nyuma y'amasaha 24 yo gukubitwa, dihydrocoumarin (1-5mM) yongereye p53 lysine 373 na acetylation 382 mu buryo bushingira ku gipimo mu turemangingo twa TK6.