Dihydrocoumarin, impumuro nziza, ikoreshwa mu biryo, ikoreshwa kandi mu gusimbuza coumarin, ikoreshwa nk'uburyohe bwo kwisiga; Kuvanga amavuta, cocout, uburyohe bwa cinnamon; Ikoreshwa kandi nk'uburyohe bw'itabi.
Ese dihydrocoumarin ni uburozi
Dihydrocoumarin ntabwo ari uburozi. Dihydrocoumarin nigicuruzwa gisanzwe kiboneka muri rinoceros yumuhondo. Itegurwa na hydrogenation ya coumarin imbere ya catalizike ya nikel kuri 160-200 ℃ kandi mukibazo. Irashobora kandi gukoreshwa nkibikoresho fatizo, hydrolyzed mumazi ya alkaline yumuti kugirango itange o-hydroxyphenylpropionic aside, dehdrasi, gufunga-gufunga byabonetse.
Imiterere y'ububiko
Ifunze kandi yijimye, ibitswe ahantu hakonje kandi humye, umwanya uri muri barrale ni mutoya bishoboka muburenganzira bwumutekano, kandi wuzuye kurinda azote. Ubike mu bubiko bukonje, buhumeka. Irinde umuriro, amazi. Ugomba kubikwa ukundi na okiside, ntukavange ububiko. Bifite ibikoresho bitandukanye hamwe nubunini bwibikoresho byumuriro.
Mu bushakashatsi bwa vitro
Muri vitro enzymatique assay, dihydrocoumarin yateje intumbero yo kubuza SIRT1 (IC50 ya 208μM). Kugabanuka mubikorwa bya SIRT1 deacetylase byagaragaye no kuri dosiye ya micromolar (85 ± 5.8 na 73 ± 13.7% ibikorwa kuri 1.6μM na 8μM). Microtubule SIRT2 deacetylase nayo yabujijwe muburyo busa na dose (IC50 ya 295μM).
Nyuma yamasaha 24 yerekanwe, dihydrocoumarin (1-5mM) yongereye cytotoxicity mumirongo ya selile TK6 muburyo buterwa na dose. Dihydrocoumarin (1-5mM) yongereye apoptose mumirongo ya selile TK6 muburyo buterwa na dose mugihe cyamasaha 6. Dose ya 5mM ya dihydrocoumarin yiyongereye apoptose kumwanya wamasaha 6 mumurongo wa selile TK6. Nyuma yamasaha 24 yo kumurika, dihydrocoumarin (1-5mM) yongereye p53 lysine 373 na 382 acetylation muburyo buterwa na dose kumurongo wa selire TK6.
Igihe cyo kohereza: Ugushyingo-01-2024